Recombinant Human TRIM5/RNF88 Protein (His Tag)

Specifications

Catalog number

PKSH033149-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tripartite motif-containing Motif 5 is a protein that in humans is encoded by the TRIM5 gene.It is a 493 amino acids protein that belongs to the TRIM/RBCC family.It contains 1 B box-type zinc finger, 1 B30.2/SPRY domain and 1 RING-type zinc finger. TRIM5 present in the cytoplasm recognizes motifs within the capsid proteins and interferes with the uncoating process, therefore preventing successful reverse transcription and transport to the nucleus of the viral genome. The exact mechanism of action has not been shown conclusively, but capsid protein from restricted viruses is removed by proteasome-dependent degradation

Abbreviation

TRIM5; RNF88

Synonyms

RING finger protein 88; RNF88; TRIM5; Tripartite motif-containing protein 5

UniProt

Q9C035

Accession Number

Q9C035

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Gln248

Field of Research

Cell biology; epigenetics and nuclear signal; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

30.8 kDa

Observed Molecular Weight

30-36 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Species

Human