Recombinant Human PRSS22/BSSP-4 Protein (His Tag)

Specifications

Catalog number

PKSH033153-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Brain-Specific Serine Protease 4 (BSSP-4) is a serine protease that preferentially cleaves the synthetic substrate H-D-Leu-Thr-Arg-pNA compared to tosyl-Gly-Pro-Arg-pNA. BSSP-4 is expressed abundantly in the epithelial cells of the airways, including trachea, esophagus and fetal lung, but scarce in adult lung and expressed at low levels in placenta, pancreas, prostate and thyroid gland. BSSP-4 belongs to the peptidase S1 family and related to trypsin, referentially hydrolyzing substrates after arginine and lysine residues. However, BSSP-4 is less susceptible to inhibition by common trypsin inhibitors such as aprotinin, α1-antitrypsin and secretory leukocyte protease inhibitor. BSSP-4 efficiently converts pro-urokinase- type plasminogen activator to its mature, active form.

Abbreviation

PRSS22; BSSP-4

Synonyms

BSSP-4; BSSP4; Brain-Specific Serine Protease 4; PRSS22; PRSS26; Serine Protease 22; Serine Protease 26; Tryptase Epsilon

UniProt

Q9GZN4

Accession Number

Q9GZN4

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala33-Ser317

Field of Research

Cell biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

31.6 kDa

Observed Molecular Weight

33-35 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM HAc-NaAc, 150mM NaCl, 10% Glycerol, pH 4.5.

Species

Human