Recombinant Human Tryptase β-2/TPSB2 Protein (His Tag)

Specifications

Catalog number

PKSH033154-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tryptases are Trypsin-like Serine Proteases. β-Tryptases are the main isoenzymes in mast cells. Βtryptases form active tetramers with heparin proteoglycan. In the tetramer, the unique arrangement of the active sites facing a narrow central pore, β-Tryptases are resistant to macromolecule protease inhibitors . When mast cells are activated, β-Tryptases are released and participate in provoking inflammatory conditions . β-Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic disorders.

Abbreviation

Tryptase β-2; TPSB2

Synonyms

TPS2; TPSB2; Tryptase Beta-2; Tryptase II; Tryptase-2

UniProt

P20231

Accession Number

AAH29356.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala19-Pro275

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

29.6 kDa

Observed Molecular Weight

30-35 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Species

Human