Recombinant Human TNFR1/TNFRSF1A Protein (Fc Tag)

Specifications

Catalog number

PKSH033160-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) is a member of the tumor necrosis factor receptor superfamily. TNFRSF1A is one of the major receptors for the tumor necrosis factor-alpha. It can activate the transcription factor NF-κB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the human genetic disorder called tumor necrosis factor associated periodic syndrome (TRAPS) or periodic fever syndrome.

Abbreviation

TNFR1; TNFRSF1A

Synonyms

TNF-R1; TNF-RI; TNFAR; TNFR-I; TNFR1; Tumor necrosis factor receptor 1; Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor type I

UniProt

P19438

Accession Number

P19438

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Leu30-Thr 211

Applications

Cell culture

Field of Research

Signal Transduction; Cell biology; Cardiovascular; Cancer; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to induce NF-κB reporter activity in HEK293 human embryonic kidney cells. Recombinant Human TNF RI inhibits a constant dose of 0.5ng/mL of Recombinant TNF alpha. The IC50 for this effect is 2.7 ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

47.2 kDa

Observed Molecular Weight

60 kDa

Species

Human