Recombinant Human USP14 Protein (His Tag)

Specifications

Catalog number

PKSH033174-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ubiquitin Carboxyl-Terminal Hydrolase 14 (USP14) belongs to the ubiquitin-specific processing (USP) family which is a deubiquitinating enzyme (DUB) with His and Cys domains. USP14 located in the cytoplasm is a proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. USP14 acts also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. In addition, USP14 is indispensable for synaptic development and function at neuromuscular junctions, required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis.

Abbreviation

USP14

Synonyms

Deubiquitinating Enzyme 14; TGT; USP14; Ubiquitin Carboxyl-Terminal Hydrolase 14; Ubiquitin Thioesterase 14; Ubiquitin-Specific-Processing Protease 14

UniProt

P54578

Accession Number

P54578

Expression System

E.coli

Tag

N-His

Sequence

Asp91-Gln494

Field of Research

Cell biology; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

48.5 kDa

Observed Molecular Weight

52 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 20% Glycerol, pH 8.0.

Species

Human