Recombinant Human UCHL1/PGP9.5 Protein (His Tag)

Specifications

Catalog number

PKSH033175-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 (UCHL1) belongs to the Peptidase C12 family. UCHL1 is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. UCHL1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity.

Abbreviation

UCHL1; PGP9.5

Synonyms

HEL-117; NDGOA; Neuron Cytoplasmic Protein 9.5; PARK5; PGP 9.5; PGP9.5; UCH-L1; UCHL1; Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1; Ubiquitin Thioesterase L1; Uch-L1

UniProt

P09936

Accession Number

P09936

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Ala223

Field of Research

Tags & Cell Markers; Cell biology; Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

25.9 kDa

Observed Molecular Weight

26 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5.

Species

Human