Recombinant Human UCHL3/UCH-L3 Protein (His Tag)

Specifications

Catalog number

PKSH033176-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ubiquitin Carboxyl-Terminal Hydrolases (UCHs) are a family of cysteine hydrolases. They catalyze the hydrolysis of amides, thioesters and esters, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin. Up regulation of UCHL3 is associated with uterine cervical neoplasms. UCHL3 is implicated in age related cognitive disorders. UCHL3 also promotes adipogenesis and insulin signaling. In mice, UCHL3 knockout have been shown to be resistant to diet-induced obesity.

Abbreviation

UCHL3; UCH-L3

Synonyms

UCH-L3; UCHL3; Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3; Ubiquitin Thioesterase L3

UniProt

P15374

Accession Number

P15374

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Ala230

Field of Research

Cell biology; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

27.3 kDa

Observed Molecular Weight

25 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 1mM DTT, 50% Glycerol, pH 8.0.

Species

Human