Recombinant Human HIP2/UBE2K Protein (His Tag, SUMO Tag)

Specifications

Catalog number

PKSH033186-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ubiquitin-Conjugating Enzyme E2 K (UBE2K) belongs to the E2 Ubiquitin-Conjugating Enzyme family. UBE2K is highly expressed in the brain; with highest levels found in cortex and striatum; and at lower levels in cerebellum and brainstem. UBE2K may mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. UBE2K is associated with the selective degradation of short-lived and abnormal proteins; such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. In addition; UBE2K is involved in Alzheimer's disease; Huntington's disease and antigen processing through its interaction with huntingtin; and MHC-heavy chain proteins.

Abbreviation

HIP2; UBE2K

Synonyms

HIP-2; HIP2; Huntingtin-Interacting Protein 2; LIG; UBE2K; Ubiquitin Carrier Protein; Ubiquitin-Conjugating Enzyme E2 K; Ubiquitin-Conjugating Enzyme E2 (25K) ; Ubiquitin-Conjugating Enzyme E2-25 kDa; Ubiquitin-Conjugating Enzyme E2-25K; Ubiquitin-Protein Ligase

UniProt

P61086

Accession Number

P61086

Expression System

E.coli

Tag

N-His-SUMO

Sequence

Met 1-Asn200

Field of Research

Cell biology; Neuroscience; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

34.5 kDa

Observed Molecular Weight

38 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 8% Sucrose, 100mM NaCl, 0.05% Tween 80, pH 7.5.

Species

Human