Recombinant Human UBA5 Protein (His Tag)

Specifications

Catalog number

PKSH033194-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

UBA5 is a member of the ubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin and ubiquitin-like proteins are recognized as covalently conjugated to various cellular substrates by a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) has a vital role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5 activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. UBA5 is located primarily in cytoplasm, while it generally localizes to the nucleus in presence of SUMO2.

Abbreviation

UBA5

Synonyms

ThiFP1; UBA5; UBE1DC1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1; Ubiquitin-like modifier-activating enzyme 5

UniProt

Q9GZZ9

Accession Number

Q9GZZ9

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Met404

Field of Research

Cell biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

47.0 kDa

Observed Molecular Weight

14&40-60 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 50mM NaCl, 1mM DTT, 10% Glycerol, pH8.0.

Species

Human