Recombinant Human UROD Protein (His Tag)

Specifications

Catalog number

PKSH033199-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Uroporphyrinogen decarboxylase (UROD), is an enzyme of the heme biosynthetic pathway which belongs to the uroporphyrinogen decarboxylase family. This enzyme is responsible for catalyzing the conversion of uroporphyrinogen to coproporphyrinogen through the removal of four carboxymethyl side chains. UROD is a homodimeric enzyme that catalyzes the fifth step in heme biosynthesis: the elimination of carboxyl groups from the four acetate side chains of uroporphyrinogen III to yield coproporphyrinogen III. Defects in UROD are the cause of familial porphyria cutanea tarda (FPCT) and hepatoerythropoietic porphyria (HEP) .

Abbreviation

UROD

Synonyms

UPD; URO-D; UROD; Uroporphyrinogen Decarboxylase

UniProt

P06132

Accession Number

P06132

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Asn367

Field of Research

Cell biology; Cardiovascular

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

43.0 kDa

Observed Molecular Weight

40 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 1mM EDTA, pH 8.0.

Species

Human