Recombinant Human UROS/UROIIIS Protein (His Tag)

Specifications

Catalog number

PKSH033200-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Uroporphyrinogen-III Synthase is an enzyme which belongs to the uroporphyrinogen-III synthase family. Uroporphyrinogen-III Synthase is ubiquitous and it is involved in Porphyrin metabolism. Porphyrins act as cofactors for a multitude of enzymes that perform a variety of processes within the cell such as Methionine synthesis (Vitamin B12) or oxygen transport (Heme) . Uroporphyrinogen-III Synthase can catalyze cyclization of the linear Tetrapyrrole, Hydroxymethylbilane, to the Macrocyclic Uroporphyrinogen III, the branch point for the various sub-pathways leading to the wide diversity of Porphyrins. Defects in Uroporphyrinogen-III Synthase are the cause of Congenital Erythropoietic Porphyria (CEP) .

Abbreviation

UROS; UROIIIS

Synonyms

Hydroxymethylbilane Hydrolyase [Cyclizing]; UROIIIS; UROS; Uroporphyrinogen-III Cosynthase; Uroporphyrinogen-III Synthase

UniProt

P10746

Accession Number

P10746

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Cys265

Field of Research

Cell biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

29.7 kDa

Observed Molecular Weight

29 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 10% Glycerol, pH 8.0.

Species

Human