Recombinant Human VEGF-A/VEGF121 Protein

Specifications

Catalog number

PKSH033207-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human VEGF121; also known as Vascular endothelial growth factor A; VEGFA; Vascular permeability factor; VPF and VEGF; is a homodimeric; heparin-binding glycoprotein which belongs to the platelet-derived growth factor (PDGF) /vascular endothelial growth factor (VEGF) family. VEGF-A is a glycosylated mitogen that specifically acts on endothelial cells and has various effects; including mediating increased vascular permeability; inducing angiogenesis; vasculogenesis; permeabilization of blood vessels and endothelial cell growth; increasing microvascular permeability; promoting cell migration and inhibiting apoptosis. Alternatively spliced transcript variants of VEGF-A encod either secreted or cell-associated isoforms. The lymphangiogenesis may be promoted by upregulation of VEGF121; which may in turn act in part via induction of VEGF-C. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors; heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways; does not activate angiogenesis and inhibits tumor growth.

Abbreviation

VEGF-A; VEGF121

Synonyms

VEGF; VEGF-A; VEGF121; VEGFA; VPF; Vascular endothelial growth factor A; Vascular permeability factor

UniProt

P15692

Accession Number

PDB: 3V2A_A

Expression System

E.coli

Tag

C-His

Sequence

Ala 14-Arg 134

Applications

Cell culture

Field of Research

Signal Transduction; Cardiovascular; Cancer; Developmental Biology; metabolism

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

15 kDa

Observed Molecular Weight

18 kDa

Species

Human