Recombinant Human ST8SIA1 Protein (His Tag)

Specifications

Catalog number

PKSH033251-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Α-N-Acetylneuraminide α-2,8-Sialyltransferase (ST8SIA1) belongs to the glycosyltransferase 29 family. ST8SIA1 is a sialytransferase that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce GD3 and GT3. ST8SIA1 is highly expressed in melanoma cell lines, adult and fetal brain, low expressed in adult and fetal lung. ST8SIA1 may act as a type II transmembrane protein with a short N-termianl cytoplasmic domain and a single-pass transmembrane domain folowed by an enzymatic domain in the lument of the Golgi apparatus.

Abbreviation

ST8SIA1

Synonyms

8-Sialyltransferase;8-Sialyltransferase 8A; Alpha-2; Alpha-N-Acetylneuraminide Alpha-2; Ganglioside GD3 Synthase; Ganglioside GT3 Synthase; SIAT8; SIAT8-A; SIAT8A; ST8SIA1; ST8SiaI; Sialyltransferase 8A; Sialytransferase St8Sia I

UniProt

Q92185

Accession Number

Q92185

Expression System

HEK293 Cells

Tag

C-His

Sequence

Tyr49-Ser356

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

36.2 kDa

Observed Molecular Weight

48 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

Species

Human