Recombinant Human β-Actin/Beta Actin Protein (His Tag)

Specifications

Catalog number

PKSH033261-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Actins are ubiquitous globular and highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. ACTB is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

Abbreviation

Β-Actin; Beta Actin

Synonyms

ACTB; Actin Cytoplasmic 1; Beta-Actin

UniProt

P60709

Accession Number

P60709

Expression System

E.coli

Tag

C-His

Sequence

Asp2-Phe375

Field of Research

Signal Transduction; Tags & Cell Markers; Isotypeloading control

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

42.8 kDa

Observed Molecular Weight

43 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 10mM Tris-HCl, 0.1% TritonX-100, 2mM DTT, 10% Glycerol, pH 8.0.

Species

Human