Recombinant Human β-Galactosidase/GLB1 Protein (His Tag)

Specifications

Catalog number

PKSH033267-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Β Galactosidase is a lysosomal β Galactosidase that hydrolyzes the terminal β Galactose from Ganglioside and Keratan sulfate. In lysosome, the mature β Galactosidase protein associates with Cathepsin A and Neuraminidase 1 to form the lysosomal multienzyme complex . An alternative splicing at the RNA level of β Galactosidase results a catalytically inactive β Galactosidase that plays an important role in vascular development. Defects of β-galactosidase (GLB1) are the cause of diseases like GM1-gangliosidosis which is a lysosomal storage disease and Morquio Syndrome B that cause patients to have abnormal elastic fibers. More than 100 mutations have been identified for β Galactosidase, which result in different residual activities of the mutant enzymes and a spectrum of symptoms in the two related diseases.

Abbreviation

Β-Galactosidase; GLB1

Synonyms

Acid Beta-Galactosidase; Beta-Galactosidase; ELNR1; Elastin Receptor 1; GLB1; Lactase

UniProt

P16278

Accession Number

P16278

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu24-Val677

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

74.6 kDa

Observed Molecular Weight

90 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

Species

Human