Recombinant Human ECH1 Protein

Specifications

Catalog number

PKSH033275-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human delta (3,5) -Delta (2,4) -dienoyl-CoA isomerase (ECH1) is a member of the hydratase/isomerase superfamily and contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. The rat orthologlocalizes to the matrix of both the peroxisome and mitochondria.It can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 plays an important role in the auxiliary step of the fatty acid beta-oxidation pathway.

Abbreviation

ECH1

Synonyms

4) -dienoyl-CoA isomerase;5) -Delta (2; Delta (3; ECH1; mitochondrial

UniProt

Q13011

Accession Number

Q13011

Expression System

E.coli

Tag

N-His

Sequence

Thr34-Leu328

Field of Research

Signal Transduction; Tags & Cell Markers; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

34.5 kDa

Observed Molecular Weight

30-35 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 5mM EDTA, pH 8.0.

Species

Human