Recombinant Human CDCP1/CD318 Protein (aa 30-341, His Tag)

Specifications

Catalog number

PKSH033302-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CUB domain-containing protein 1 (CDCP1) is a transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains; and a smaller intracellular domain (ICD) containing five tyrosines. CDCP1 is widely expressed in human epithelial tissues; but its phosphorylation is only seen in mitotically detached or shedding cells; consistent with its role in the negative regulation of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsinor EDTA; or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype.

Abbreviation

CDCP1; CD318

Synonyms

CD318; CUB domain-containing protein 1; Membrane glycoprotein gp140; SIMA135; Subtractive immunization M plus HEp3-associated 135 kDa protein; TRASK; Transmembrane and associated with src kinases

UniProt

Q9H5V8

Accession Number

Q9H5V8-3

Expression System

HEK293 Cells

Tag

C-His

Sequence

Phe30-Ser341

Field of Research

Tags & Cell Markers, Stem Cells, Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

36.1 kDa

Observed Molecular Weight

55 kDa

Species

Human