Recombinant Human UBE2D4 Protein (GST Tag)

Specifications

Catalog number

PKSH033309-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ubiquitin-Conjugating Enzyme E2 D4 (UBE2D4) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2D4 has been proposed to participate in Ubl conjugation pathway. UBE2D4 takes part in post-translational protein modification; protein K6-linked ubiquitination; protein K11-linked ubiquitination; protein K27-linked ubiquitination; protein K29-linked ubiquitination; protein K48-linked ubiquitination; and protein K63-linked ubiquitination. UBE2D4 regulate of protein metabolic process. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro; UBE2D4 able to promote polyubiquitination using all 7 ubiquitin Lys residues; but may prefer 'Lys-11' and 'Lys-48'-linked poly-ubiquitination.

Abbreviation

UBE2D4

Synonyms

HBUCE1; UBCH5D; UBE2D4; Ubiquitin Carrier Protein D4; Ubiquitin-Conjugating Enzyme E2 D4; Ubiquitin-Protein Ligase D4

UniProt

Q9Y2X8

Accession Number

Q9Y2X8

Expression System

E.coli

Tag

N-GST

Sequence

Met 1-Met 147

Field of Research

Cell biology; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

43.5 kDa

Observed Molecular Weight

40 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.

Species

Human