Recombinant Human SUMO1 Protein (His Tag)

Specifications

Catalog number

PKSH033311-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Small Ubiquitin-Related Modifier 1 (SUMO1) is an Ubiquitin-like protein that belongs to the ubiquitin family with SUMO subfamily. It is a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. SUMO1 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO1 is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. SUMO1 is not active until the last four amino acids of the carboxy-terminus are cleaved off. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins and may also regulate a network of genes involved in palate development.

Abbreviation

SUMO1

Synonyms

DAP1; GAP-Modifying Protein 1; GMP1; OFC10; SENP2; SMT3; SMT3 Homolog 3; SMT3C; SMT3H3; SUMO-1; SUMO1; Sentrin; Small Ubiquitin-Related Modifier 1; Smt3C; UBL1; Ubiquitin-Homology Domain Protein PIC1; Ubiquitin-Like Protein; Ubiquitin-Like Protein SMT3C

UniProt

P63165

Accession Number

AAH66306

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Val101

Field of Research

Cell biology; Cardiovascular; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

13.7 kDa

Observed Molecular Weight

17-19 kDa

Species

Human