Recombinant Human SERPINB9/PI-9 Protein (Human Cells, His Tag)

Specifications

Catalog number

PKSH033318-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serpin B9; also known as Cytoplasmic antiproteinase 3 (CAP-3) ; is a cytoplasm protein which belongs to the large superfamily of serine proteinase inhibitors (serpins) ; which bind to and inactivate serine proteinases. Serpin B9 is an inhibitor of the granzyme B/perforin lytic pathway. It is expressed in normal mammary epithelial cells but not in most mammary carcinoma cell lines. These interactions are involved in many cellular processes; including coagulation; fibrinolysis; complement fixation; matrix remodeling; and apoptosis. Serpin-B9 expression in immune-privileged cells; APCs; and CTLs protects these cells against the actions of granzyme B; and when expressed in tumor cells or virally infected hepatocytes; confers resistance to killing by CTL and NK cells. Expression of increasing levels of Serpin-B9 in target cells may progressively inhibit immune surveillance by blocking NK and CTL-induced cytotoxicity through the perforin / granzyme pathway and then through the Fas / FasL pathway.

Abbreviation

SERPINB9; PI-9

Synonyms

CAP3; Cytoplasmic antiproteinase 3; PI-9; Peptidase inhibitor 9; Serpin B9

UniProt

P50453

Accession Number

P50453

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met 1-Pro376

Field of Research

Cell biology; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43.4 kDa

Observed Molecular Weight

35-40 kDa

Species

Human