Recombinant Human UBE2T Protein (His Tag)

Specifications

Catalog number

PKSH033331-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ubiquitin-Conjugating Enzyme E2 T (UBE2T) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2T accepts the ATP-dependent ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro; UBE2T is able to catalyze polyubiquitination using all 7 ubiquitin Lys residues; but may prefer 'Lys-11'-; 'Lys-27'-; 'Lys-48'- and 'Lys-63'-linked polyubiquitination. UBE2T is an important factor of the Faconi anemia pathway of DNA damage repair and; upon self-inactivation; may negatively regulate the Faconi pathway.

Abbreviation

UBE2T

Synonyms

Cell Proliferation-Inducing Gene 50 Protein; UBE2T; Ubiquitin Carrier Protein T; Ubiquitin-Conjugating Enzyme E2 T; Ubiquitin-Protein Ligase T

UniProt

Q9NPD8

Accession Number

Q9NPD8

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Val197

Field of Research

Cell biology; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

24.7 kDa

Observed Molecular Weight

25-30 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.

Species

Human