Recombinant Human Butyrylcholinesterase/BCHE Protein (His Tag)

Specifications

Catalog number

PKSH033346-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Butyrylcholine Esterase (BCHE) is a secreted protein that belongs to the type-B carboxylesterase/lipase family. BCHE is a major acetylcholine hydrolyzing enzyme in the circulation. It is detected in blood plasma and present in most cells except erythrocytes. BCHE is an esterase with broad substrate specificity. BCHE can contribute to the inactivation of the neurotransmitter acetylcholine. BCHE can degrade a large number of neurotoxic organophosphate esters. Thus, it plays important pharmacological and toxicological roles and is thought to be involved in the pathological progression. Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency) which is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine and other ester local anesthetics.

Abbreviation

Butyrylcholinesterase; BCHE

Synonyms

Acylcholine Acylhydrolase; BCHE; Butyrylcholine Esterase; CHE1; Choline Esterase II; Cholinesterase; Pseudocholinesterase

Accession Number

P06276

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu29-Leu602

Field of Research

Signal Transduction; Neuroscience; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

66.1 kDa

Observed Molecular Weight

90 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.

Species

Human