Recombinant Human UBE2D1 Protein (GST Tag)

Specifications

Catalog number

PKSH033367-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ubiquitin-conjugating enzyme E2 D1 (UBE2D1) belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes; or E1s; ubiquitin-conjugating enzymes; or E2s; and ubiquitin-protein ligases; or E3s. This enzyme is closely related to a stimulator of iron transport (SFT) ; and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Abbreviation

UBE2D1

Synonyms

SFT; Stimulator of Fe transport; UBC4/5 homolog; UBC5A; UBCH5; UBCH5A; UbcH5; Ubiquitin carrier protein D1; Ubiquitin-conjugating enzyme E2 D1; Ubiquitin-conjugating enzyme E2 (17) KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; Ubiquitin-protein ligase D1

UniProt

P51668

Accession Number

P51668

Expression System

E.coli

Tag

N-GST

Sequence

Met 1-Met 147

Field of Research

Cell biology; epigenetics and nuclear signal

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

42.9 kDa

Observed Molecular Weight

40 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.

Species

Human