Recombinant Human GPNMB Protein (aa 22-486, His Tag)

Specifications

Catalog number

PKSH033371-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Osteoactivin is an intracellular glycoprotein belongs to the NMB/pMEL-17 family; which is asscociated with cell endosomal/lysomal compartments. Human Osteoactivin is a 560 amino acid type I transmembrane protein; and one alternate splice form shows a 12 amino acid insert between amino acid 339-340. An additional 206 amino acid isoform shows a mutation at position 181 that results in a 26 amino acid substitution for the C-terminal 380 amino acids. Cells knowns to express Osteoactivin include fibroblast; osteoblasts; myeloid dendritic cell; melanocytes; plus fetal chondrocytes and stratum basale keratinocytes; macrophages/keratinocytes.

Abbreviation

GPNMB

Synonyms

GPNMB; HGFIN; NMB; Osteoactivin; Transmembrane Glycoprotein HGFIN; Transmembrane Glycoprotein NMB

UniProt

Q14956

Accession Number

Q14956

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala22-Pro486

Field of Research

Immunology, Cancer

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

53.1 kDa

Observed Molecular Weight

80-120 kDa

Species

Human