Recombinant Human S100A8&S100A9 Heterodimer Protein (His Tag)

Specifications

Catalog number

PKSH033381-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

S100A8 is a member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca2+-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. Calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase.

Abbreviation

S100A8&S100A9 Heterodimer

Synonyms

S100A8/S100A9 Heterodimer

UniProt

P05109; P06702

Accession Number

P05109&P06702

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Glu93 (S100A8) &Thr 2-Pro114 (S100A9)

Field of Research

Signal Transduction; Cell biology; Neuroscience; Cancer; epigenetics and nuclear signal; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

11.7&13.2 kDa

Observed Molecular Weight

13&15 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM HEPES, 10% Glycerol, 150mM NaCl, 2.5mM EDTA, pH 7.4.

Species

Human