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Product details

Recombinant Human CD3 ε/CD3E Protein (His Tag)

Catalog number PKSH033388-01

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Price for PKSH033388-01

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Size / count 10 µg

Specifications

Catalog number
PKSH033388-01
Size / count
10 µg
Dry ice
No
Hazardous
No
Discontinued
No
Background
T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) . TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Abbreviation
CD3 ε; CD3E
Synonyms
CD3 epsilon; CD3E; CD3e; IMD18; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; T-Cell Surface Glycoprotein CD3 Epsilon Chain; T3E
UniProt
P07766
Accession Number
P07766
Expression System
HEK293 Cells
Tag
C-His
Sequence
Asp23-Asp126
Applications
ELISA
Field of Research
Immunology; Stem cells
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Immobilized Human CD3E-His at 10 ug/ml (100 ul/wel) can bind Human Anti-CD3 mAb. The ED50 of Recombinant Human Anti-CD3 mAb is 3-15 ng/ml.
Reconstitution
Please refer to the printed manual for detailed information.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
12.8 kDa
Observed Molecular Weight
15-21 kDa
Species
Human

Frequently Asked Questions

What is the catalog number for Recombinant Human CD3 ε/CD3E Protein (His Tag)?

The catalog number for Recombinant Human CD3 ε/CD3E Protein (His Tag) is PKSH033388.

What sizes are available for Recombinant Human CD3 ε/CD3E Protein (His Tag)?

Recombinant Human CD3 ε/CD3E Protein (His Tag) is available in the following size(s): 10 µg, 50 µg.

How many pack sizes does Recombinant Human CD3 ε/CD3E Protein (His Tag) come in?

Recombinant Human CD3 ε/CD3E Protein (His Tag) is available in 2 different pack sizes.

Is Recombinant Human CD3 ε/CD3E Protein (His Tag) in stock?

Recombinant Human CD3 ε/CD3E Protein (His Tag) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Recombinant Human CD3 ε/CD3E Protein (His Tag)?

Recombinant Human CD3 ε/CD3E Protein (His Tag) is manufactured by Elabscience.

What is the estimated delivery time for Recombinant Human CD3 ε/CD3E Protein (His Tag)?

Estimated delivery time for Recombinant Human CD3 ε/CD3E Protein (His Tag) is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Recombinant Human CD3 ε/CD3E Protein (His Tag)?

The safety data sheet for Recombinant Human CD3 ε/CD3E Protein (His Tag) is available here: https://789.bio/eb/mHGmn1.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

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