Recombinant Human HtrA2/Omi Protein (His Tag)

Specifications

Catalog number

PKSH033408-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

High temperature requirement protein A2 (HTRA2) is a single-pass membrane protein .It contains 1 PDZ (DHR) domain and belongs to the peptidase S1C family. HtrA2 can be released from the mitochondria during apoptosis and uses its four most N-terminal amino acids to mimic a caspase and be recruited by IAP caspase inhibitors such as XIAP and CIAP1/2. It promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. The protein cleaves THAP5 and promotes its degradation during apoptosis.

Abbreviation

HtrA2; Omi

Synonyms

HTRA2; High temperature requirement protein A2; HtrA2; OMI; Omi stress-regulated endoprotease; PRSS25; Serine protease 25; Serine protease HTRA2; Serine proteinase OMI; mitochondrial

UniProt

O43464

Accession Number

O43464

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala134-Glu458

Field of Research

Cell biology; Neuroscience; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

36.0 kDa

Observed Molecular Weight

35-43 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 4mM HCl.

Species

Human