Recombinant Human Kininogen 1/KNG1 Protein (His Tag)

Specifications

Catalog number

PKSH033413-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Kininogen-1 is a secreted protein which contains three cystatin domains. There are two alternatively spliced forms, designated as the high molecular weight (HMW) and low MW (LMW) forms. Kininogen-1 plays a critical role in blood coagulation and inflammatory response. Kininogens are inhibitors of thiol proteases. Kininogen-1 participates in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII, also inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. The active peptide bradykinin that is released from Kininogen-1 shows a variety of physiological effects: influence in smooth muscle contraction, induction of hypotension, natriuresis and diuresis, decrease in blood glucose level. It is a mediator of inflammation and causes increase in vascular permeability, stimulation of nociceptors release of other mediators of inflammation. It has a cardioprotective effect. LMW-kininogen inhibits the aggregation of thrombocytes and doesn’t involved in blood clotting.

Abbreviation

Kininogen 1; KNG1

Synonyms

BDK; Fitzgerald Factor; HMWK; High Molecular Weight Kininogen; KNG; KNG1; Kininogen-1; Williams-Fitzgerald-Flaujeac Factor; lpha-2-Thiol Proteinase Inhibitor

Accession Number

P01042-2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gln19-Ser427

Field of Research

Signal Transduction; Cardiovascular

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

46.9 kDa

Observed Molecular Weight

61 kDa

Species

Human