Recombinant Human OX40/TNFRSF4 Protein (His Tag)

Specifications

Catalog number

PKSH033416-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

OX40, also termed CD134 and TNFRSF4, is a T cell co-stimulatory molecule of the TNF receptor superfamily which plays a key role in the survival and homeostasis of effector and memory T cells. OX40 is expressed on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7. The interaction between OX40 and OX40 ligand (OX40L) will occur when activated T cells bind to professional antigen-presenting cells (APCs) . The T-cell functions, including cytokine production, expansion, and survival, are then enhanced by the OX40 costimulatory signals. OX40 signals are critical for controlling the function and differentiation of Foxp3+ regulatory T cells. OX40-OX40L interaction regulates T-cell tolerance, peripheral T-cell homeostasis, and T-cell-mediated inflammatory diseases.

Abbreviation

OX40; TNFRSF4

Synonyms

ACT35; CD134; IMD16; OX40; TNFRSF4; TXGP1L; Tumor necrosis factor receptor superfamily member 4; Txgp1

Accession Number

P43489

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu29-Ala216

Applications

ELISA

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized Mouse OX40L-His at 10μg/ml (100 μl/well) can bind Human OX40-6His.*: Biotinylated by NHS-biotin prior to testing The ED50 of Recombinant Human OX40-6His (Cat#CK60) is 1.44 ug/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

21.0 kDa

Observed Molecular Weight

38-45 kDa

Species

Human