Recombinant Human CD23 Protein (His Tag)

Specifications

Catalog number

PKSH033432-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Low affinity immunoglobulin epsilon Fc receptor (CD23) is a secreted and single-pass type II membrane protein which is also exists as a soluble excreted form. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. Unlike many of the antibody receptors, CD23/FCER2 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. In flow cytometry, CD23/FCER2 is helpful in the differentiation of chronic lymphocytic leukemia (CD23-positive) from mantle cell leukemia (CD23-negative) . CD23/FCER2 can also be demonstrated in germinal centre B-cells using immunohistochemistry, but it is not present in the resting cells of the surrounding mantle zone. CD23/FCER2 has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specificantigen) .

Abbreviation

CD23

Synonyms

BLAST-2; C-type lectin domain family 4 member J; CD23; CD23A; CLEC4J; FCE2; FCER2; Fc-epsilon-RII; IGEBF ; Immunoglobulin E-binding factor; Low affinity immunoglobulin epsilon Fc receptor; Lymphocyte IgE receptor

UniProt

P06734

Accession Number

P06734

Expression System

HEK293 Cells

Tag

N-His

Sequence

Asp48-Ser321

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

32.1 kDa

Observed Molecular Weight

35-40 kDa

Species

Human