Recombinant Human E-Cadherin/CDH1 Protein (His Tag)

Specifications

Catalog number

PKSH033437-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

E-Cadherin is a classical member of the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats; a transmembrane region; and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric; breast; colorectal; thyroid; and ovarian cancers. Loss of function is thought to contribute to progression in cancer by increasing proliferation; invasion; and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells; and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. Also; E-Cadherin has a potent invasive suppressor role and it is a ligand for integrin alpha-E/beta-7.

Abbreviation

E-Cadherin; CDH1

Synonyms

Arc-1 ; CAM 120/80; CD324; CDH1; CDHE; Cadherin-1; E-Cadherin; E-cad; E-cadherin; ECAD; LCAM; UVO

UniProt

P12830

Accession Number

P12830

Expression System

HEK293 Cells

Tag

C-His

Sequence

Asp155-Ile707

Field of Research

Signal Transduction; Tags & Cell Markers; Cancer; Developmental Biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

61.2 kDa

Observed Molecular Weight

80-90 kDa

Species

Human