Recombinant Human HOXB4 Protein (His Tag)

Specifications

Catalog number

PKSH033457-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Homeobox B4 (HOXB4) is encoded by the HOXB4 gene which is a member of the the class I homeobox (HOX) gene family and encodes a nuclear protein with a homeobox DNA-binding domain. These genes are master control regulators of developmental programs including embryonic and adult hematopoiesis. Multiple HOX genes, including HOXB4, are highly expressed in the hematopoietic stem cells (HSC) compartment. HOXB4 gene can act in opposite ways when expressed by different cells, promoting the proliferation of stem cells whilst activating the apoptotic pathway in some embryonic structures. The protein HOXB4, as a homeodomain transcription factor, has been shown to be an important regulator of stem cell renewal and hematopoiesis. Incellular or ectopic expression of HOXB4 expands hematopoietic stem and progenitor cells in vivo and in vitro, making it a potential candidate for therapeutic stem cell expansion.

Abbreviation

HOXB4

Synonyms

HOX2F; HOXB4; Homeobox protein Hox-2.6; Homeobox protein Hox-2F; Homeobox protein Hox-B4

UniProt

P17483

Accession Number

P17483

Expression System

E.coli

Tag

N-His

Sequence

Met1-Leu251 (Leu175Asp, Glu176Lys, Glu178Lys)

Field of Research

Epigenetics and nuclear signal; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

29.8 kDa

Observed Molecular Weight

36 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 4mM HCl.

Species

Human