Recombinant Human CLDN18.2/Claudin-18 A2 Protein (His Tag)

Specifications

Catalog number

PKSH033470-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Claudin-18 (CLDN18) is a protein that in humans is encoded by the CLDN18 gene. It belongs to the group of claudins. CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells. CLDN18 plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. CLDN18 has two isoform A1 and isoform A2. IMAB362 (Claudiximab) is a monoclonal antibody against isoform 2 of Claudin-18. It is under investigation for the treatment of gastrointestinal adenocarcinomas and pancreatic tumors. IMAB362 was developed by Ganymed Pharmaceuticals AG.

Abbreviation

CLDN18.2; Claudin-18 A2

Synonyms

CLDN18; Claudin-18

UniProt

P56856

Accession Number

P56856-2

Expression System

E.coli

Tag

N-His

Sequence

Asp28-Leu76

Field of Research

Signal Transduction

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

19.7 kDa

Observed Molecular Weight

18 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 25mM NaCl, 0.1% Triton X-100, 10% glycerol, 0.1mM GSH, 0.01mM GSSG, pH 8.0.

Species

Human