Recombinant Human VEGF165/VEGFA Protein

Specifications

Catalog number

PKSH033475-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human Vascular endothelial growth factor (VEGF) ; also known as VEGF-A and vascular permeability factor (VPF) ; belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms; of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.

Abbreviation

VEGF165; VEGFA

Synonyms

MVCD1; VEGF; VEGF165; VPF; Vascular Endothelial Growth Factor Isoform 165

UniProt

P15692

Accession Number

P15692-4

Expression System

HEK293 Cells

Tag

None

Sequence

Ala27-Arg191

Applications

ELISA

Field of Research

Cardiovascular, Signal Transduction, Cancer, Developmental Biology, Kits, Lysates, Other, Metabolism

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

50

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.1 kDa

Observed Molecular Weight

18-22 kDa

Species

Human