Recombinant Human DUSP3/VHR Protein (His Tag)

Specifications

Catalog number

PKSH033476-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human DUSP3 belongs to the dual specificity protein phosphatase subfamily. DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. DUSPs are major modulators of critical signalling pathways that are dysregulated in various diseases. They negatively regulate members of the mitogen-activated protein kinase superfamily; which are associated with cellular proliferation and differentiation. DUSP3 is expressed in human tissues including breast and ovarian.DUSP3 shows activity both for tyrosine-protein phosphate and serine-protein phosphate; but displays a strong preference toward phosphotyrosines. Human DUSP3 specifically dephosphorylates and inactivates ERK1 and ERK2.

Abbreviation

DUSP3; VHR

Synonyms

DUSP3; Dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; VHR; Vaccinia H1-related phosphatase

UniProt

P51452

Accession Number

P51452

Expression System

E.coli

Tag

N-His

Sequence

Ser2-Pro185

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

22.6 kDa

Observed Molecular Weight

18-22 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Species

Human