Recombinant Human TNF-alpha/TNFA Protein

Specifications

Catalog number

PKSH033490-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

TNFα is a homotrimer with a subunit molecular mass of 17 kD cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation; differentiation; inflammation; viral replication; tumorigenesis; autoimmune diseases and in viral; bacterial; fungal; and parasitic infections. Besides inducing hemorrhagic necrosis of tumors; TNF was found to be involved in tumorigenesis; tumor metastasis; viral replication; septic shock; fever; inflammation; and autoimmune diseases including Crohn’s disease; and rheumatoid arthritis as well as graft-versus-host disease.

Abbreviation

TNF-alpha; TNFA

Synonyms

Cachectin; TNF; TNF-Alpha; TNF-a; TNFA; TNFSF2; Tumor Necrosis Factor; Tumor Necrosis Factor Ligand Superfamily Member 2

UniProt

P01375

Accession Number

P01375

Expression System

E.coli

Tag

None

Sequence

Val77-Leu233

Applications

Cell culture

Field of Research

Signal Transduction; Cardiovascular; Cancer; immunology; metabolism

Endotoxin

< 0.01 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-50 pg/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

17.5 kDa

Observed Molecular Weight

16 kDa

Species

Human