Recombinant Human Transferrin Receptor/TFRC Protein (His Tag)

Specifications

Catalog number

PKSH033496-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Transferrin receptor protein 1 (TFRC) belongs to the peptidase M28 family that is synthesized as a 172 amino acid (aa) . TFRC regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. It binds one transferrin or HFE molecule per subunit and binds the HLA class II histocompatibility antigen, DR1. It Interacts with SH3BP3 and STEAP3, facilitates TFRC endocytosis in erythroid precursor cells. Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. It positively regulates T and B cell proliferation through iron uptake.

Abbreviation

Transferrin Receptor; TFRC

Synonyms

CD71; T9; TFR; TR; TRFR; TfR; Transferrin receptor protein 1; Trfr; p90

UniProt

P02786

Accession Number

P02786

Expression System

HEK293 Cells

Tag

N-His

Sequence

Leu101-Phe760

Field of Research

Cardiovascular; Cancer; immunology; metabolism; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

74.9 kDa

Observed Molecular Weight

80-90 kDa

Species

Human