Recombinant Human TNFSF14/LIGHT Protein (mFc Tag)

Specifications

Catalog number

PKSH033497-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human TNFSF14 Protein; also known as LIGHT; belongs to a member of the tumor necrosis factor (TNF) ligand family. It can bind to NFRSF3/LTBR. It is a ligand for TNFRSF14; which is a member of the tumor necrosis factor receptor superfamily; and it is also known as a herpesvirus entry mediator ligand (HVEML) . TNFSF14 encodes a protein with a 37 aa cytoplasmic domain; 21aa transmembrane domain and 182 aa extracellular region. The gene is predominantly expressed in the spleen and also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart; placenta; liver; lung; appendix; and kidney; and no expression seen in fetal tissues; endocrine glands; or nonhematopoietic tumor lines. TNFSF14 protein was found to probably function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. Studies have shown that this protein can prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.

Abbreviation

TNFSF14; LIGHT

Synonyms

CD258; HVEM-L; HVEML; Herpes virus entry mediator ligand; LIGHT; LTg; TNFSF14; TR2; Tumor necrosis factor ligand superfamily member 14

UniProt

O43557

Accession Number

AAC39563.1

Expression System

HEK293 Cells

Tag

N-mFc

Sequence

Ile84-Val240

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43.3 kDa

Observed Molecular Weight

45-60 kDa

Species

Human