Recombinant Human Thrombospondin-1/THBS1 Protein (His Tag)

Specifications

Catalog number

PKSH033500-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Thrombospondin-1 (TSP-1) is a 150-180kDa calcium-sensitive protein that is secreted as a disulfide-linked homotrimer. TSP-1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM) . TSP-1 contains an N-terminal Laminin G-like globular domain, an extended central region with one vWFC domain, 3 TSP type 1domains, 2 EGF-like domains, and 8 TSP type3 domains, and a globular TSP C-terminal domain. Distinct regions of TSP-1 have been associated with binding to particular ECM or cellular molecules. TSP-1 counteracts the angiogenic, hypotensive, and antithrombotic effects of nitric oxide (NO) . It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC. TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF-beta and inducing release of the active cytokine from the latency associated peptide (LAP) .

Abbreviation

Thrombospondin-1; THBS1

Synonyms

THBS1; TSP; TSP1; Thrombospondin-1

UniProt

P07996

Accession Number

P07996

Expression System

HEK293 Cells

Tag

C-His

Sequence

Asn19-Pro1170

Field of Research

Cardiovascular; Neuroscience; Cancer; metabolism; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

129.2 kDa

Observed Molecular Weight

130&170 kDa

Citation 01

https://www.sciencedirect.com/science/article/pii/S0945053X22000397

Species

Human