Recombinant Human FGF-2/FGF basic/FGFb Protein (aa 1-155) (Q65I, C96S, N111G)

Specifications

Catalog number

PKSH033502-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro; including mitogenesis; cellular migration; differentiation; angiogenesis; and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media; without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable; daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule; so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies; and maintained cell growth; pluripotency and differentiation potential with a 2-day feeding schedule.

Abbreviation

FGF-2; FGF basic; FGFb

Synonyms

Basic fibroblast growth factor; FGF-2; FGF2; FGFB; Fibroblast growth factor 2; HBGF-2; Heparin-binding growth factor 2; bFGF

UniProt

P09038

Accession Number

BAG70264.1

Expression System

E.coli

Tag

None

Sequence

Met1-Ser155

Field of Research

Cardiovascular, Signal Transduction, Neuroscience, Stem Cells, Cancer, Developmental Biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

17.2 kDa

Observed Molecular Weight

16 kDa

Species

Human