Recombinant Human FGF-2/FGF basic/FGFb Protein

Specifications

Catalog number

PKSH033503-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro; including mitogenesis; cellular migration; differentiation; angiogenesis; and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media; without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable; daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule; so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies; and maintained cell growth; pluripotency and differentiation potential with a 2-day feeding schedule.

Abbreviation

FGF-2; FGF basic; FGFb

Synonyms

Basic fibroblast growth factor; FGF-2; FGF2; FGFB; Fibroblast growth factor 2; HBGF-2; Heparin-binding growth factor 2; bFGF

UniProt

P09038

Accession Number

BAG70135.1

Expression System

E.coli

Tag

None

Sequence

Met1-Ser155

Field of Research

Cardiovascular, Signal Transduction, Neuroscience, Stem Cells, Cancer, Developmental Biology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

17.2 kDa

Observed Molecular Weight

17 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 7.5.

Species

Human