Recombinant Human CD4/LEU3 Protein (His Tag)

Specifications

Catalog number

PKSH033506-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD4 is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. Mature human CD4 consists of a 371 amino acid extracellular region containing four immunoglobulin-like domains; a 22 aa transmembrane segment; and a 40 aa cytoplasmic domain. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. CD4 binds directly to MHC class II molecules on antigen presenting cells (10) . This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and; in human; as a co-receptor for the gp120 surface glycoprotein of HIV-1.

Abbreviation

CD4; LEU3

Synonyms

CD4; CD4mut; Scd4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

UniProt

P01730

Accession Number

P01730

Expression System

HEK293 Cells

Tag

C-His

Sequence

Lys26-Trp390

Field of Research

Immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

41.7 kDa

Observed Molecular Weight

58 kDa

Species

Human