Recombinant Human SIGLEC5 Protein (His & Flag & Fc)

Specifications

Catalog number

PKSH033530-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human Siglec-5 are Itype (Igtype) lectins belonging to the Ig superfamily; They are characterized by an N terminal Ig-like V type domain which mediates sialic acid binding; followed by varying numbers of Ig-like C2 type domains. SIGLEC5 has also been designated CD170; they are expressed by monocytic or myeloid lineage cells; and also found at high levels in peripheral blood leukocytes; spleen; bone marrow and at lower levels in lymph node; lung; appendix; placenta; pancreas and thymus. SIGLEC5 are expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. Siglec5 to 11 share a high degree of sequence similarity with CD33/Siglec3 both in their extracellular and intracellular regions. They are collectively referred to as CD33 related Siglecs. One remarkable feature of the CD33 related Siglecs is their differential expression pattern within the hematopoietic system This fact; together with the presence of two conserved immunoreceptor tyrosinebased inhibition motifs (ITIMs) in their cytoplasma tails; suggests that CD33 related Siglecs are involved in the regulation of cellular activation within the immune system.

Abbreviation

SIGLEC5

Synonyms

CD170; CD33 antigen-like 2; CD33L2; OB-BP2; OBBP; OBBP2; Obesity-binding protein 2; SIGLEC-5; SIGLEC5; Sialic acid-binding Ig-like lectin 5; Siglec-5

UniProt

O15389

Accession Number

O15389

Expression System

HEK293 Cells

Tag

C-His-Flag-Fc

Sequence

Glu17-Thr434

Field of Research

Immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

74.1 kDa

Observed Molecular Weight

90-110 kDa

Species

Human