Recombinant Human FLT-3/FLK-2 Protein (Fc Tag)

Specifications

Catalog number

PKSH033543-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2and Stk-1is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

Abbreviation

FLT-3; FLK-2

Synonyms

CD135; FL Cytokine Receptor; FLK-2; FLK2; FLT-3; FLT3; Fetal Liver Kinase-2; Fms-Like Tyrosine Kinase 3; Receptor-Type Tyrosine-Protein Kinase FLT3; STK-1; STK1; Stem Cell Tyrosine Kinase 1

UniProt

P36888

Accession Number

AAI26351.1

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Asn27-Asn541

Field of Research

Signal Transduction; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

85.3 kDa

Observed Molecular Weight

120 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Species

Human