Recombinant Human PSGL-1/CD162 Protein (Fc Tag)

Specifications

Catalog number

PKSH033546-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PSGL-1 (CD162), is a mucintype glycoprotein that plays a key role in leukocyte adhesion. Human PSGL-1 cDNA encodes 412 amino acids (aa) . It expressed on neutrophils, monocytes and most lymphocytes. The mature PSGL-1 (aa 42-412) is expressed as a disulfide-linked homodimer that signals intracellularly and promotes integrin activation. PSGL-1 is found on virtually all leukocytes, dendritic cells, platelets, and some endothelial cells. It is primarily responsible for early events in extravasation, especially rolling adhesion of leukocytes to vascular endothelium. Through high affinity, This SLe (x) -type proteoglycanPGSL-1 calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.

Abbreviation

PSGL-1; CD162

Synonyms

CD162; CLA; P-selectin glycoprotein ligand 1; PSGL-1; PSGL1; SELPLG; Selectin P ligand

UniProt

Q14242

Accession Number

Q14242

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Gln42-Gly295

Field of Research

Cardiovascular; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

52.9 kDa

Observed Molecular Weight

100-130 kDa

Species

Human