Recombinant Human BDNF Protein (with pro peptide)

Specifications

Catalog number

PKSH033559-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis; whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival; growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects; Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.

Abbreviation

BDNF

Synonyms

Abrineurin; BDNF; Brain-Derived Neurotrophic Factor

UniProt

P23560

Accession Number

P23560

Expression System

E.coli

Tag

None

Sequence

Ala19-Arg247 (R125A, R127A, R128A)

Field of Research

Cardiovascular; Neuroscience; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

25.6 kDa

Observed Molecular Weight

28 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM PB, 10% Trehalose, 100mM NaCl, 0.02% Tween 80, pH 6.0.

Species

Human