Recombinant Human Noggin/NOG Protein (aa 28-232, Fc Tag)

Specifications

Catalog number

PKSH033577-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs) . Mature Human Noggin contains an N-terminal acidic region; a central basic heparin-binding segment and a C-terminal cysteine-knot structure. Noggin is very highly conserved among vertebrates; such that mature human Noggin shares 99%; 99%; 98%; 97% and 89% aa sequence identity with mouse; rat bovine; equine and chicken Noggin; respectively. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung; skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions; addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state; or alternatively; to differentiate into dopaminergic neurons.

Abbreviation

Noggin; NOG

Synonyms

Noggin; SYM1; SYNS1

UniProt

Q13253

Accession Number

Q13253

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Gln28-Cys232

Field of Research

Developmental Biology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

50.2 kDa

Observed Molecular Weight

60 kDa

Species

Human