Recombinant Human CD10/Neprilysin Protein (His Tag)

Specifications

Catalog number

PKSH033583-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Neprilysin/CD10 (NEP) is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The functions is both as an endopeptidase with a thermolysin-like specificity and as a dipeptidyl-carboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide. NEP has also been identified as the common acute lymphocytic leukemia antigen (CALLA), and is expressed on the surface of lymphocytes in some disease states. These and other observations have resulted in considerable interest in NEP as a target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid β peptide (Aβ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting Aβ accumulation.

Abbreviation

CD10; Neprilysin

Synonyms

SCA; Neutral endopeptidase 24.; CALLA; CD10; CMT2T; NEP; SCA43; SFE; MME; Atriopeptidase; Common acute lymphocytic leukemia antigen; Enkephalinase; Neprilysin; Neutral endopeptidase; Neutral endopeptidase 24.11; Skin fibroblast elastase; EPN; NEP; gp100; CD10 antigen; CD10) ; DKFZp686O16152; EC 3.4.24.11; enkephalinase) ; Membrane metallo endopeptidase; Membrane metallo endopeptidase (neutral endopeptidase; Membrane metallo endopeptidase variant 1; Membrane metallo endopeptidase variant 2; Membrane metalloendopeptidase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10; Membrane metalloendopeptidase variant 1; Membrane metalloendopeptidase variant 2; membrane-associated; MGC126681; MGC126707; neprilysin-390; neprilysin-411

UniProt

P08473

Accession Number

P08473

Expression System

HEK293 Cells

Tag

N-His

Sequence

Tyr52-Trp750

Field of Research

Cancer; immunology; Stem cells

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

80.9 kDa

Observed Molecular Weight

94-100 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Species

Human