Recombinant Human VAP-1/AOC3 Protein (Fc Tag)

Specifications

Catalog number

PKSH033590-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Membrane primary amine oxidase (AOC3) ; also known as vascular adhesion protein (VAP-1) and HPAO; this protein is a member of the semicarbazide-sensitive amine oxidase (SSAO) family. VAP-1 is a type 1 membrane-bound glycoprotein that has a distal adhesion domain and an enzymatically active amine oxidase site outside of the membrane; VAP-1 has adhesive properties; functional monoamine oxidase activity; and possibly plays a role in glucose handling; leukocyte trafficking; and migration during inflammation. This rise in metabolic products contributes to generating advanced glycation end-products and oxidative stress along with the monoamine detoxification in the organism. It is highly expressed on the endothelium of the lung and trachea; and absent from leukocytes and epithelial cells. Membrane-bound VAP-1 releases an active; soluble form of the protein; which may be conducive to increased inflammation and the progression of many vascular disorders. In particular; elevation of VAP-1 activity and the increased enzymatic-mediated deamination is proposed to play a role in renal and vascular disease; oxidative stress; acute and chronic hyperglycemia; and diabetes complications.

Abbreviation

VAP-1; AOC3

Synonyms

AOC3; Copper amine oxidase; HPAO; Membrane primary amine oxidase; SSAO; Semicarbazide-sensitive amine oxidase; VAP-1; VAP1; Vascular adhesion protein 1

UniProt

Q16853

Accession Number

Q16853

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Arg28-Asn763

Field of Research

Signal Transduction; Neuroscience

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

108.5 kDa

Observed Molecular Weight

120 kDa

Species

Human