Recombinant Human LILRB4/CD85k/ILT3 Protein (aa 22-259, Fc Tag)

Specifications

Catalog number

PKSH033604-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4 (LILRB4/CD85k/ILT3) is an approximately transmembrane glycoprotein that negatively regulates immune cell activation. Mouse LILRB4 consists of a 215 amino acid (aa) extracellular domain with two Ig-like domains; a 22 aa transmembrane segment; and a 75 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM) . Within the ECD; mouse LILRB4 shares 45% and 77% aa sequence identity with human and rat LILRB4; respectively. Alternative splicing of mouse LILRB4 generates a potentially soluble isoform that lacks the transmembrane segment. LILRB4 is expressed on dendritic cells (DC) ; monocytes; macrophages; and vascular endothelial cells (EC) . Ligation of LILRB4 triggers ITIM-mediated inhibition of cellactivating signaling; leading to enhanced immune tolerance and reduced allogeneic graft rejection. Soluble LILRB4 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL. In turn; CD8+ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes; DC; and EC.

Abbreviation

LILRB4; CD85k; ILT3

Synonyms

CD85k; Gp49b; Leukocyte immunoglobulin-like receptor subfamily B member 4; Lilrb4; Mast cell surface Lyophilized from a 0.2 μm filtered solution of PBS; pH 7.4.glycoprotein Gp49B

UniProt

Q8NHJ6

Accession Number

Q8NHJ6

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Gln22-Glu259

Field of Research

Cardiovascular; epigenetics and nuclear signal; immunology

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

53.2 kDa

Observed Molecular Weight

65-85 kDa

Species

Human